RNA

Part:BBa_I1030:Design

Designed by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton   Group: Antiquity   (2003-01-31)


RNA Regulation Target ( Promoter driven by C0040 and cI mod #1 CDS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_I1030 Custom cI Protein with TetR Promoter is based on BioBricks BBa_C0050 and BBa_R0040. It is designed such that BBa_I1031 and BBa_I1032can be used to interfere with the BBa_1030 transcript by anti-sense mRNA binding, in the KISS or micRNA method shown below. BBa_I1030 and BBa_I1031 or BBa_I1032 can act indepently of related biobrick parts BBa_I1041, BBa_I1042, BBa_I1020, BBa_BBa_I1023 and BBa_1013. [<A href="http://biobricks.ai.mit.edu/BB_References.htm#KEIL96">KEIL96</A>]

Incompatible with systems containing IPTG, or cI.

May be some cross talk with systems containing BBa_I1010. Compatible with all other BBa_line I-line parts.

Proper operation requires AMP/CAP complex, which should normally be present in the cell.



Source

see references

References